斑馬魚視黃醛脫氫酶2基因（raldh2）的啟動子活性分析及其受視黃酸調控的反饋抑制機制.pdf

1、南京師范大學碩士學位論文斑馬魚視黃醛脫氫酶2基因（raldh2）的啟動子活性分析及其受視黃酸調控的反饋抑制機制姓名：張媚申請學位級別：碩士專業(yè)：遺傳學指導教師：許曉風趙慶順20080501望壹室塹蔓奎堂堡圭蘭些望莖整塑●__l__●_●____●___l____●●________●I__●_●_●_●_l__l____●___I_______。。。。。?！灰灰灰籄nalysisofZebrafishraldh2Pro

2、moterrevealingthattherepressionofraldh2byRAIsMediatedbyRAREsintheFirstIntronABSTRACTRALDI12isthemainRetinoicacid∞qsynthesizingenzymeduringdevelopmentandisthemostwidelyexpressedinundifferentiatedsomites，limbbudsandvarious

3、organsTheexpressionofRALDH2geneisrepressedbyRAHoweverthemechanismofthisrepressionisstillunknownPreviousstudiessuggestthatraldh2isadirectRAresponsegene，andraldh2expressionisdecreasedwithRAtreatmentAnalysesoftheraldh2promo

4、terincelltransfeetionsprovedthatRAdirectlyrepressesitsactivityThesedatademonstratethatthereisafeedbackmechanismregulatingproductionofRANeverthelessthepromoterthatWaschosentod0activityassaydidnotshowniceactivityPerforming

5、bioinformaticsanalysiSwedidnotfindanyrctinoicacidresponseelements(RAREs)inthebasicpromoterabout27kbupstreamofthetranscriptionstartsiteWhereas，somepotentialcandidateRAREsarefoundinthefirstinlxonInthisstudywemadesomepromot

6、erconstructscontainingthebasicpromoterandthefragmentsfromtheintron1，whicharelocatedfrom27kbupstreamofthetranscriptionstartsitetoabout2kbdownstreamandfrom13573to15115Byperformingdualluciferaseassayofthefragmentscontaining

7、thoseRAREs，wefoundthatRAinducesastronginhibitionoftheexpressionofraldh2TheseresultssuggestthatthepotentailRAREswhicharclocatedintheintron1from13573to15115，mediatetherepressiveeffectofRAAdditionallywefoundthatthefeedbackl